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Cloning cylinders improve colony isolation by assuring a homogenous culture of cells rather than a mixed population. Polystyrene construction Assure a homogenous culture of cells Cylinders are tapered in shape for wider bottomTo isolate incubated colonies, a cloning cylinder is placed around the colony of choice and attached by sterile grease. After adding trypsin into the cylinder, the dish is placed into an incubator until cells detach. Cells are then collected and transferred to another vessel. Cylinders are tapered so the bottom edge is wider than the top to afford a good seal. Molded of polystyrene. Ordering information: Individual sizes includes 5 sterile bags of 10. Assorted pack includes 4 sterile bags of 10 of each size, 12 bags total.

These cloning cylinders facilitate the isolation of individual cell clones, each generated from a single cell, yielding a homogenous population of cells. To isolate a clone, a thin layer of grease is applied to the bottom edge of the cylinder and then inverted over the clone of choice. After adding a small volume of Trypsin or EDTA, the dish is incubated at 37ºC (98.6ºF) briefly until cells detach. Cells are then collected from inside the cylinder and transferred to another vessel for further growth. _x000D_ Polystyrene cloning cylinders are supplied in sterile packs. Three sizes: 4.7mm I.D. x 8mm H, 6.4mm I.D. x 8mm H and 9.5mm I.D. x 11mm H. Each cylinder is tapered so the bottom edge is wider than the top ensuring a good sealing surface. Packed 10 per bag, one size. This listing is for pkg. of 50. Technical Specification Item Cloning Cylinders Material Polystyrene Size Medium 1/4 in ID x 5/16 in H Color Clear This item is restricted for international sale.