Trade Name: Amicon. Key Applications: Protein Purification, Western Blotting and Protein Extraction. Application Notes: Fixed angle rotor: 14,000 x g for concentration spin; 1,000 x g for reverse spin. Length, cm (in): 4.99 (0.196). Filter Material: Regenerated Cellulose. Centrifugal Application: Protein Concentration and Desalting. Centrifugal Filter Unit Type: Protein Sample Prep. Color Code: Clear. Media Brand Name: Ultracel. Minimum Final Concentrate Volume,?L: 15. Filter Type: Ultrafiltration. Length,mm: 49.9. NMWL, kDa: 100. Replaces: 42424. Diameter,mm: 10.8. Filter Code: YML. Volume, mL: 0.5. Filtration Area, cm squared: 1. Device Material: Housing: Styrene/butadiene and Cap and Tube: Polypropylene. Material Supplied: The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample. Maximum initial sample volume,?L: 500. Typical final concentration volume,?L: 15 - 20. Recommended relative centrifugal force: 14,000 x g for concentration spin; 1,000 x g for reverse spin. Maximum relative centrifugal force: 15,000 x g. Active membrane area, cm squared: 1. Hold up volume,?L: <=1. Filter device: Copolymer styrene/butadiene. Membrane: Millipore Ultracell low-binding regenerated cellulose. Collection tube: Polypropylene. The ultrafiltration membranes in Amicon Ultra-0.5 mL devices contain trace amounts of the following:: Amicon Ultra 3K devices: glycerine; Amicon Ultra 10K device: triethylene glycol (TEG). If these materials interfere with analysis, pre-rinse the device with buffer or Milli-Q water. If interference continues, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q water. Filter device: Diameter: 9.4mm and 29.6mm Length. Tube: 10.8mm Diameter and 43.0mm Length. Filter device and tube: 49.9mm Length (concentration mode; device in tube); 37.3mm Length (reverse spin; device upside down in tube).