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Promega Tnt(r) Sp6 Quick Coupled 1sys L2080 FREE S&H . Promega Genomic libraries.

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Brand name, Manufacturer name, BrandPromega
ManufacturerPromega Promega Corporation
Extra Product NamePromega Tnt(r) Sp6 Quick Coupled 1sys L2080 FREE S&H . Promega Genomic libraries.
moq, multiple, tierMinQty1, Number_of_items1
MPN, Keyword, PartNumber, Part_numberL2080
qtyInStock, Hazmat0
SKUSF9-VW-L2080-EA
tierPrice11159.00
AdultProduct, Autographed, Memorabilia, TradeInEligibleFalse
AsinB075DK89XF
Aspecttransportation_regulatory_class
Bullet_pointChoice of SP6 or T7 promoter Functional protein is expressed in one hour, ready for use without further purification Single-Tube, Coupled Transcription/Translation for Eukaryotic Cell-Free Protein Expression Use protein for protein:protein and protein:nucleic acid interactions, protein modification and more
ClassificationId7656083011
DisplayNameNatural Proteins
Height, Width1024 500 75
ItemClassificationBASE_PRODUCT
ItemName, Item_namePromega TNT SP6 Quick Coupled Transcription/Translation System, 40 Reactions
Item_type_keywordscience-lab-natural-proteins
Linkhttps://m.media-amazon.com/images/I/41abmSgAkuL._SL75_.jpg https://m.media-amazon.com/images/I/41abmSgAkuL.jpg https://m.media-amazon.com/images/I/51uCFnzXtgL.jpg
ProductTypePROTEIN_SUPPLEMENT_POWDER
Product_descriptionThe TNT Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TNT Quick Master Mix. The TNT Quick Coupled Transcription/Translation System is available in two configurations for the expression of genes cloned downstream from either the T7 or SP6 RNA polymerase promoters. To use these cell-free expression systems, 0.2-2.0ug of circular plasmid DNA containing a T7 or SP6 promoter, or a PCR-generated fragment containing a T7 promoter, is added to an aliquot of the TNT Quick Master Mix and incubated in a 50ul reaction volume for 60-90 minutes at 30 degrees C. The expression reaction produces significant quantities of protein for a variety of applications including GST pull-downs and gel shift assays.
Product_site_launch_date2017-09-05T19:58:46.359Z
VariantMAIN
WebsiteDisplayGrouphealth_and_beauty_display_on_website
WebsiteDisplayGroupNameHealth and Beauty

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Promega TNT SP6 Quick Coupled Transcription/Translation System, 40 Reactions
Brand: Promega
The TNT Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TNT Quick Master Mix. The TNT Quick Coupled Transcription/Translation System is available in two configurations for the expression of genes cloned downstream from either the T7 or SP6 RNA polymerase promoters. To use these cell-free expression systems, 0.2-2.0ug of circular plasmid DNA containing a T7 or SP6 promoter, or a PCR-generated fragment containing a T7 promoter, is added to an aliquot of the TNT Quick Master Mix and incubated in a 50ul reaction volume for 60-90 minutes at 30 degrees C. The expression reaction produces significant quantities of protein for a variety of applications including GST pull-downs and gel shift assays.
Details
  • Single-Tube, Coupled Transcription/Translation for Eukaryotic Cell-Free Protein Expression
  • Functional protein is expressed in one hour, ready for use without further purification
  • Use protein for protein:protein and protein:nucleic acid interactions, protein modification and more
  • Choice of SP6 or T7 promoter
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