Storage: ?20?C UNSPSC Code: 41105600 Components: Enzyme Solution; SuRE/Cut Buffer L 10x concentrated RIDADR: NONH for all modes of transport Analysis Note: Activity in PCR buffer: Not tested Application: Ksp I belongs to the class of "rare-cutter" enzymes, which are useful for generating large fragments of genomic DNA with 3G??-cohesive ends. General description: Average fragment size Bacterial genomic DNA: Average Ksp I fragments are 20 to 50kb. Isoschizomers Ksp I is an isoschizomer of Sac II and Sst II. Methylation sensitivity In contrast to its isoschizomer Sac II, Ksp I is not sensitive to the presence of 5?-methylcytosine at the 5?-C residue of the recognition sequence. Ligation and recutting assay Ksp I fragments obtained by complete digestion of 1?g ?DNA are ligated with 1U T4 DNA Ligase in a volume of 10?L by incubation for 16 hours at +4?C in 66mM Tris-HCl, 5mM MgCl 2 , 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20?C) resulting in >90% recovery of 1?g ?DNA ? Ksp I fragments. Subsequent re-cutting with Ksp I yields >90% of the typical pattern of ?DNA ? Ksp I fragments. Other Notes: For life science research only. Not for use in diagnostic procedures. Quality: Absence of nonspecific endonuclease activities 1?g ?DNA is incubated for 16 hours in 50?L SuRE/Cut Buffer L with an excess of Ksp I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5?g [ 3 H] labeled calf thymus DNA are incubated with 3?L Ksp I for 4 hours at +37?C in a total volume of 100?L 50mM Tris-HC

Storage: ?20?C UNSPSC Code: 41105600 Components: Enzyme Solution; SuRE/Cut Buffer L 10x concentrated RIDADR: NONH for all modes of transport Analysis Note: Activity in PCR buffer: Not tested Application: Ksp I belongs to the class of "rare-cutter" enzymes, which are useful for generating large fragments of genomic DNA with 3G??-cohesive ends. General description: Average fragment size Bacterial genomic DNA: Average Ksp I fragments are 20 to 50kb. Isoschizomers Ksp I is an isoschizomer of Sac II and Sst II. Methylation sensitivity In contrast to its isoschizomer Sac II, Ksp I is not sensitive t