{"v":"2.0","page_type":"product","title":"Storage: ?20?C UNSPSC Code: 41105600 Components: Enzyme Solution; SuRE/Cut Buffer L 10x concentrated RIDADR: NONH for all modes of transport Analysis Note: Activity in PCR buffer: Not tested Application: Ksp I belongs to the class of \"rare-cutter\" enzymes, which are useful for generating large fragments of genomic DNA with 3G??-cohesive ends. General description: Average fragment size Bacterial genomic DNA: Average Ksp I fragments are 20 to 50kb. Isoschizomers Ksp I is an isoschizomer of Sac II and Sst II. Methylation sensitivity In contrast to its isoschizomer Sac II, Ksp I is not sensitive to the presence of 5?-methylcytosine at the 5?-C residue of the recognition sequence. Ligation and recutting assay Ksp I fragments obtained by complete digestion of 1?g ?DNA are ligated with 1U T4 DNA Ligase in a volume of 10?L by incubation for 16 hours at +4?C in 66mM Tris-HCl, 5mM MgCl 2 , 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20?C) resulting in >90% recovery of 1?g ?DNA ? Ksp I fragments. Subsequent re-cutting with Ksp I yields >90% of the typical pattern of ?DNA ? Ksp I fragments. Other Notes: For life science research only. Not for use in diagnostic procedures. Quality: Absence of nonspecific endonuclease activities 1?g ?DNA is incubated for 16 hours in 50?L SuRE/Cut Buffer L with an excess of Ksp I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5?g [ 3 H] labeled calf thymus DNA are incubated with 3?L Ksp I for 4 hours at +37?C in a total volume of 100?L 50mM Tris-HC","breadcrumbs":[{"label":"Home","href":"/python/html6/index.html"},{"label":"Brands","href":"/python/html6/menu_brand_index/menu_brand_index_brands_87101ba214.html"},{"label":"Microflex","href":"/python/html6/menu_brand_products/1000/104000/menu_brand_products_Microflex_1_cf4d075731.html"},{"label":"B003Z58KJK","href":null}],"content":{"product":{"mpn":"B003Z58KJK","manufacturer":"Microflex","brand":"Microflex","brand_url":"/python/html6/menu_brand_products/1000/104000/menu_brand_products_Microflex_1_cf4d075731.html","title":"Storage: ?20?C UNSPSC Code: 41105600 Components: Enzyme Solution; SuRE/Cut Buffer L 10x concentrated RIDADR: NONH for all modes of transport Analysis Note: Activity in PCR buffer: Not tested Application: Ksp I belongs to the class of \"rare-cutter\" enzymes, which are useful for generating large fragments of genomic DNA with 3G??-cohesive ends. General description: Average fragment size Bacterial genomic DNA: Average Ksp I fragments are 20 to 50kb. Isoschizomers Ksp I is an isoschizomer of Sac II and Sst II. Methylation sensitivity In contrast to its isoschizomer Sac II, Ksp I is not sensitive to the presence of 5?-methylcytosine at the 5?-C residue of the recognition sequence. Ligation and recutting assay Ksp I fragments obtained by complete digestion of 1?g ?DNA are ligated with 1U T4 DNA Ligase in a volume of 10?L by incubation for 16 hours at +4?C in 66mM Tris-HCl, 5mM MgCl 2 , 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20?C) resulting in >90% recovery of 1?g ?DNA ? Ksp I fragments. Subsequent re-cutting with Ksp I yields >90% of the typical pattern of ?DNA ? Ksp I fragments. Other Notes: For life science research only. Not for use in diagnostic procedures. Quality: Absence of nonspecific endonuclease activities 1?g ?DNA is incubated for 16 hours in 50?L SuRE/Cut Buffer L with an excess of Ksp I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis. Absence of exonuclease activity Approximately 5?g [ 3 H] labeled calf thymus DNA are incubated with 3?L Ksp I for 4 hours at +37?C in a total volume of 100?L 50mM Tris-HC","description":"","attributes":[{"name":"Brand name","values":[{"value":"Microflex","url":"/python/html6/menu_attr_pair/1000/136000000/136953000/menu_attr_pair_Brandname_Microflex_1_ff430059a6.html"}],"name_url":"/python/html6/menu_attr_pair/1000/136000000/136953000/menu_attr_pair_Brandname_Microflex_1_ff430059a6.html"},{"name":"Manufacturer name","values":[{"value":"Microflex","url":"/python/html6/menu_attr_pair/1000/162000000/162195000/menu_attr_pair_Manufacturername_Microflex_1_b5024b66a2.html"}],"name_url":"/python/html6/menu_attr_pair/1000/162000000/162195000/menu_attr_pair_Manufacturername_Microflex_1_b5024b66a2.html"},{"name":"Manufacturer","values":[{"value":"Microflex","url":"/python/html6/menu_attr_pair/1000/145000000/145713000/menu_attr_pair_Manufacturer_Microflex_1_6e2919b77d.html"}],"name_url":"/python/html6/menu_attr_pair/1000/145000000/145713000/menu_attr_pair_Manufacturer_Microflex_1_6e2919b77d.html"},{"name":"dataSource","values":[{"value":"amazonScraper","url":"/python/html6/menu_attr_pair/1000/58000000/58817000/menu_attr_pair_dataSource_amazonScraper_1_5cb8c9f3c5.html"}],"name_url":"/python/html6/menu_attr_pair/1000/58000000/58817000/menu_attr_pair_dataSource_amazonScraper_1_5cb8c9f3c5.html"},{"name":"moq","values":[{"value":"1","url":"/python/html6/menu_attr_pair/1000/164000000/164262000/menu_attr_pair_moq_1_1_29ca727a89.html"}],"name_url":"/python/html6/menu_attr_pair/1000/164000000/164262000/menu_attr_pair_moq_1_1_29ca727a89.html"},{"name":"multiple","values":[{"value":"1","url":"/python/html6/menu_attr_pair/1000/257000000/257999000/menu_attr_pair_multiple_1_1_2f60c1bab8.html"}],"name_url":"/python/html6/menu_attr_pair/1000/257000000/257999000/menu_attr_pair_multiple_1_1_2f60c1bab8.html"},{"name":"qtyInStock","values":[{"value":"0","url":"/python/html6/menu_attr_pair/1000/119000000/119332000/menu_attr_pair_qtyInStock_0_1_38510c9e21.html"}],"name_url":"/python/html6/menu_attr_pair/1000/119000000/119332000/menu_attr_pair_qtyInStock_0_1_38510c9e21.html"},{"name":"SKU","values":[{"value":"B003Z58KJK","url":"/python/html6/menu_attr_pair/1000/48000000/48183000/menu_attr_pair_SKU_B003Z58KJK_1_cc58546c88.html"}],"name_url":"/python/html6/menu_attr_pair/1000/48000000/48183000/menu_attr_pair_SKU_B003Z58KJK_1_cc58546c88.html"},{"name":"tierMinQty1","values":[{"value":"1","url":"/python/html6/menu_attr_pair/1000/226000000/226987000/menu_attr_pair_tierMinQty1_1_1_974840705d.html"}],"name_url":"/python/html6/menu_attr_pair/1000/226000000/226987000/menu_attr_pair_tierMinQty1_1_1_974840705d.html"}],"images":[],"distributors":[{"name":"Amazon","sku":"B003Z58KJK","url":"https://www.amazon.com/dp/B003Z58KJK","pricing":[],"stock":{"available":false,"quantity":null,"lead_time_days":null},"moq":1,"multiple":1}],"amazon":{"available":false,"products":[]},"metadata":{"generated_date":"2026-09-17","last_updated":"2026-09-17","data_sources":["Amazon"],"completeness":0.65,"version":"1.2"}}},"pagination":null,"nav":{"home":"/python/html6/index.html","brands":"/python/html6/menu_brand_index/menu_brand_index_brands_87101ba214.html","parts":"/python/html6/menu_part_index/menu_part_index_parts_909e6bf0ca.html","attributes":"/python/html6/menu_attr_index/menu_attr_index_attributes_d7f67a250d.html"}}