Storage: ?20?C UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Features and Benefits: ?Maximize viability and yield of isolated cells with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content. ?Count on higher specific activity of the enzyme blend as a result of higher Collagenase I + II purity (determined by HPLC analysis). ?Obtain higher experimental reproducibility due to higher lot-to-lot consistency. Application: Liberase ? DL (Dispase Low) Research Grade has been used for the isolation of skin stem cells from mice. General description: Liberase DH Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other, and with a high concentration of Dispase ? , a non-clostridial neutral protease. Liberase DL (Dispase Low) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold. Other Notes: For life science research only. Not for use in diagnostic procedures. Preparation Note: Stabilizers: Calcium Working concentration: Liberase Research Grade Enzyme Working Concentration Liberase enzymes have significantly higher specific activities than traditional collagenases. This means that the working concentration of Liberase Research Grade Purified Enzymes, expressed in mg/ml, will be lower than that of traditional collagenase. When the application is on the Roche list of applications at www.collagenase.com, use the Liberase Research Grade concentration recommended

Storage: ?20?C UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Features and Benefits: ?Maximize viability and yield of isolated cells with an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content. ?Count on higher specific activity of the enzyme blend as a result of higher Collagenase I + II purity (determined by HPLC analysis). ?Obtain higher experimental reproducibility due to higher lot-to-lot consistency. Application: Liberase ? DL (Dispase Low) Research Grade has been used for the isolation of skin stem cells from mice. General des