The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample. Trade Name: Amicon. Key Applications: Protein Purification, Western Blotting, Protein Extraction. Application Notes: Fixed angle rotor: 14,000 x g for concentration spin; 1,000 x g for reverse spin. Length, cm (in): 4.99 (0.196). Filter Material: Regenerated Cellulose. Centrifugal Application: Protein Concentration and Desalting. Centrifugal Filter Unit Type: Protein Sample Prep. Color Code: Clear. Media Brand Name: Ultracel. Minimum Final Concentrate Volume, ?L: 15. Filter Type: Ultrafiltration. Length, mm: 49.9. NMWL, kDa: 10. Diameter, mm: 10.8. Filter Code: PLGCD. Volume, mL: 0.5. Filtration Area, cm squared: 1. Device Material: Housing: Styrene/butadiene, Cap and Tube: Polypropylene.
The Isopore membrane is a polycarbonate, track-etched screen filter recommended for all analyses in which the sample is viewed on the surface of the membrane. Isopore membrane offers distinct advantages for the analysis of airborne contaminants and other particles using optical or electron microscopy. The Isopore membrane is composed of polycarbonate film, which has a smooth, glass-like surface for clearer sample observation. The unique manufacturing process of the membrane ensures a precise pore diameter and a consistent pore size for accurate separation of samples by size. Isopore membranes do not stain resulting in low background interference. Clearing is not necessary for most transmitted light microscopy. Matched-weight filters are not usually required because of low, constant tar and ash weights. Isopore membranes are non-hygroscopic, which permits rapid drying and reduced sample analysis time. Trade Name: Isopore. Filter Material: Polycarbonate. Media Brand Name: Isopore. Refractive Index: 1.6. Bubble Point at 23 degree C: =7.1 bar, air with water. Filter Type: Screen filter. Pore Size (?m): 0.1. Wettability: Hydrophilic. Filter Diameter,mm: 142. Filter Code: VCTP. Filtration Device and Accessory Type: Filter Discs/Sheets. Filter Color: White. Filter Surface: Plain. Thickness,?m: 25-30. Gravimetric Extractables, %: <=1. Porosity %: 4.18. Chemotaxis, bioassays, cytology, air monitoring: Water Flow Rate (mL/min/cm squared): 0.5, Air Flow Rate (L/min/cm squared): 1.5.
Trade Name: Amicon. Key Applications: Protein Purification, Western Blotting and Protein Extraction. Application Notes: Fixed angle rotor: 14,000 x g for concentration spin; 1,000 x g for reverse spin. Length, cm (in): 4.99 (0.196). Filter Material: Regenerated Cellulose. Centrifugal Application: Protein Concentration and Desalting. Centrifugal Filter Unit Type: Protein Sample Prep. Color Code: Clear. Media Brand Name: Ultracel. Minimum Final Concentrate Volume,?L: 15. Filter Type: Ultrafiltration. Length,mm: 49.9. NMWL, kDa: 100. Replaces: 42424. Diameter,mm: 10.8. Filter Code: YML. Volume, mL: 0.5. Filtration Area, cm squared: 1. Device Material: Housing: Styrene/butadiene and Cap and Tube: Polypropylene. Material Supplied: The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample. Maximum initial sample volume,?L: 500. Typical final concentration volume,?L: 15 - 20. Recommended relative centrifugal force: 14,000 x g for concentration spin; 1,000 x g for reverse spin. Maximum relative centrifugal force: 15,000 x g. Active membrane area, cm squared: 1. Hold up volume,?L: <=1. Filter device: Copolymer styrene/butadiene. Membrane: Millipore Ultracell low-binding regenerated cellulose. Collection tube: Polypropylene. The ultrafiltration membranes in Amicon Ultra-0.5 mL devices contain trace amounts of the following:: Amicon Ultra 3K devices: glycerine; Amicon Ultra 10K device: triethylene glycol (TEG). If these materials interfere with analysis, pre-rinse the device with buffer or Milli-Q water. If interference continues, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q water. Filter device: Diameter: 9.4mm and 29.6mm Length. Tube: 10.8mm Diameter and 43.0mm Length. Filter device and tube: 49.9mm Length (concentration mode; device in tube); 37.3mm Length (reverse spin; device upside down in tube).